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KMID : 1098420150230010020
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Korean Journal of Medicinal Crop Science
2015 Volume.23 No. 1 p.20 ~ p.26
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Anti-oxidant and Inhibitory Activity on NO Production of Extract and its Fractions from Rosa davurica Pall. Leaves
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Song Jong-Ho
Lee Sun-Ryung
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Abstract
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In the present study, we investigated biological activities of Rosa davurica Pall. leaves in order to evaluate the possibility as a natural biomaterial. The 80% ethanol extract and its subsequent fractions of Rosa davurica Pall. leaves were prepared using several solvents with different polarities. The extraction yield was 33.4, 36.6, 25.2, 18.7, 5.8 and 5.8% in ethanol extract, aqueous, butanol, ethyl acetate, n-hexane, chloroform fractions, respectively. The ethyl acetate fraction (661.38 ㎎/g), butanol fraction (396.68 ㎎/g) and 80% ethanolic extract (239.54 ㎎/g) has higher total polyphenol contents than other fractions. The antioxidant activity was detected in ethanolic extract, ethyl acetate and butanol fractions. The ethyl acetate fraction showed the highest levels of DPPH radical scavenging activity (IC50, 4.77 ㎍/㎖). Moreover, the ethyl acetate fraction significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results indicate that 80% ethanol extract and its fractions of Rosa davurica Pall. leaf, especially ethyl acetate fraction, have the properties of anti-oxidant and anti-inflammation, suggesting leaf of Rosa davurica Pall. may be a candidate for natural and functional materials.
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KEYWORD
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Rosa davurica, Antioxidant, Anti-Inflammation, Nitric Oxide
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